The two main forms of adipose tissue (AT) are white adipose tissue (WAT), which functions primarily as an energy reservoir, and brown adipose tissue (BAT), which contributes to thermoregulation. AT lipidomics analyses have reported differences between BAT and WAT and demonstrated remodeling upon exercise or cold exposure. However, these studies applied generic methods of lipid extraction and measurement rather than techniques tailored to these tissues. Grzybek, Palladini, et al. present a shotgun lipidomics method for the analysis for ATs with an unprecedented coverage of more than 300 lipid species. With their method, they not only observe clear differences between BAT and WAT lipidomes, but also amongst WAT subtypes, i.e. gonadal and inguinal subcutaneous ATs.