Enteroendocrine cells (EECs) are gastrointestinal epithelial cells that produce more than twenty different hormones. The physiological role of the large number of EECs in the large intestine is not clear. Billing, Larraufie, et al. studied EECs of the large intestine using single cell RNA-sequencing. They identified different subpopulations and showed that these likely represent cellular gradients mapping along the proximal-distal and crypt-surface gut axes. Their data suggests that these cells can contribute to circulating gut hormone concentrations despite their distal location.