Cover Story Current Issue
Epidemiological evidences provide proof of concept that certain pesticides are involved in metabolic disorders, but also in the pathophysiology of Parkinson's disease (PD). In addition, large prospective cohort studies reported that type 2 diabetes (T2D) and PD are epidemiologically associated, including an elevated risk of developing PD in patients with T2D.
Current Issue
A distinct vagus-beta cell neural circuit senses glucose and modulates insulin secretion
A distinct vagus-beta cell neural circuit senses glucose and modulates insulin secretion
Objective
Vagal sensory neurons (VSN) convey peripheral glycemic information to the brain, yet the specific pathways that continuously sense glucose fluctuations and regulate hormone secretion and feeding remain poorly defined. Here, we examined the anatomical and functional aspects of an integrated circuit originating in pancreatic β-cells, projecting through the nodose ganglion, and engaging the dorsal vagal complex to relay feedback to β-cells.
Methods
We performed monosynaptic viral fluorescent tracing, RNA sequencing, RNAscope, chemogenetics, optogenetics, neuronal silencing, automated glucose telemetry, feeding assays, neural activity measurements, glucose sensing, and intracellular calcium measurements using 2-photon microscopy.
Results
The vagal transcriptome exhibited metabolic state- and diet-dependent regulation of pathways involved in glucose sensing, insulin secretion, and glucose homeostasis. Viral tracing identified abundant VSN innervating β-cells, including a subset expressing cocaine- and amphetamine-regulated transcript (VSNCART), whose activity was modulated by metabolic state and altered brainstem neuronal activity. VSNCART stimulation increased acetylcholine and C-peptide secretion and lowered blood glucose in a metabolic state-dependent manner, whereas silencing impaired glucose-stimulated insulin secretion and induced glucose intolerance. VSNCART activation suppressed food intake, while inhibition increased feeding, also in a metabolic state-dependent manner. C-Fos labeling and two-photon Ca2+ imaging revealed that VSNCART neurons exhibit dose-dependent excitatory responses to glucose.
Conclusions
We identified a vagal sensory neuron–β-cell circuit and showed that VSNCART neurons sense glucose to regulate insulin secretion, feeding behavior, and glucose homeostasis.
Graphical abstract
How the brain detects glycemic fluctuations to integrate hormone secretion and feeding behavior is unclear. Kumar et al. describe a vagus-brain-β-cell circuit identifying the vagus as a glucose-sensing hub. They show that a population of glucose excitatory vagal sensory neurons communicate with β-cells and the brain to regulate insulin secretion, feeding behavior and glucose homeostasis.
Articles in Press
A distinct vagus-beta cell neural circuit senses glucose and modulates insulin secretion
A distinct vagus-beta cell neural circuit senses glucose and modulates insulin secretion
Objective
Vagal sensory neurons (VSN) convey peripheral glycemic information to the brain, yet the specific pathways that continuously sense glucose fluctuations and regulate hormone secretion and feeding remain poorly defined. Here, we examined the anatomical and functional aspects of an integrated circuit originating in pancreatic β-cells, projecting through the nodose ganglion, and engaging the dorsal vagal complex to relay feedback to β-cells.
Methods
We performed monosynaptic viral fluorescent tracing, RNA sequencing, RNAscope, chemogenetics, optogenetics, neuronal silencing, automated glucose telemetry, feeding assays, neural activity measurements, glucose sensing, and intracellular calcium measurements using 2-photon microscopy.
Results
The vagal transcriptome exhibited metabolic state- and diet-dependent regulation of pathways involved in glucose sensing, insulin secretion, and glucose homeostasis. Viral tracing identified abundant VSN innervating β-cells, including a subset expressing cocaine- and amphetamine-regulated transcript (VSNCART), whose activity was modulated by metabolic state and altered brainstem neuronal activity. VSNCART stimulation increased acetylcholine and C-peptide secretion and lowered blood glucose in a metabolic state-dependent manner, whereas silencing impaired glucose-stimulated insulin secretion and induced glucose intolerance. VSNCART activation suppressed food intake, while inhibition increased feeding, also in a metabolic state-dependent manner. C-Fos labeling and two-photon Ca2+ imaging revealed that VSNCART neurons exhibit dose-dependent excitatory responses to glucose.
Conclusions
We identified a vagal sensory neuron–β-cell circuit and showed that VSNCART neurons sense glucose to regulate insulin secretion, feeding behavior, and glucose homeostasis.
Graphical abstract
How the brain detects glycemic fluctuations to integrate hormone secretion and feeding behavior is unclear. Kumar et al. describe a vagus-brain-β-cell circuit identifying the vagus as a glucose-sensing hub. They show that a population of glucose excitatory vagal sensory neurons communicate with β-cells and the brain to regulate insulin secretion, feeding behavior and glucose homeostasis.
Registration & Abstract Submission are open!
13th
Helmholtz Diabetes Conference
Munich, 21-23. Sep 2026
2024 impact factor: 6.6
You are what you eat
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