A Genetic Screen Identifies Crat As A Regulator Of Pancreatic Beta-Cell Insulin Secretion

Dassine Berdous, Xavier Berney, Ana Rodriguez Sanchez-Archidona, Maxime Jan, ... Bernard Thorens

Glucose-stimulated insulin secretion is a critical function in the regulation of glucose homeostasis and its deregulation is associated with the development of type 2 diabetes. Here, we performed a genetic screen using islets isolated from the BXD panel of advanced recombinant inbred (RI) lines of mice to search for novel regulators of insulin production and secretion.


We identified Crat as a regulator of insulin secretion whose action is mediated by an effect on total cellular insulin content; this effect also depends on the genetic background of the RI mouse lines. These data also show that in the presence of the stimulatory conditions used the insulin secretion rate is directly related to the insulin content.